山羊 anti-Pig IgG (Heavy & Light Chain) Antibody (Biotin)
ELISA, IHC, WB
Polyclonal
IgG
Biotin
1 image
产品编号 ABIN101877
Supplier Product No.: 614-1602
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-Pig IgG (Heavy & Light Chain) Antibody (Biotin) (ABIN101877)
抗原
IgG
克隆类型
多克隆
标记
Biotin
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- Pig
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宿主
- 山羊
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Supplier Product No.
- 614-1602
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Supplier
- Rockland
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原理
- Swine IgG (H&L) Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Swine IgG and Swine Serum.
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产品特性
- Anti-Swine IgG whole molecule antibody generated in goat detects specifically Swine IgG whole molecule. This secondary antibody anti-Swine is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose.
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免疫原
- Optional[Immunogen]: Swine IgG whole molecule
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Goat anti-Pig IgG (Heavy & Light Chain) antibody (FITC)
WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
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应用备注
- Application Note: Anti-Swine IgG (H&L) Biotin Conjugated Antibody has been tested by western blot and assayed against 1.0 μg of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:150,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Anti-Swine IgG (H&L) generated in goat detects swine Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG is conjugated to Biotin.
抗原
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