山羊 anti-小鼠 IgG (Fc Region) Antibody (HRP)
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北京 101111
Quick Overview for 山羊 anti-小鼠 IgG (Fc Region) Antibody (HRP) (ABIN101860)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Fc Region
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 210-1303
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Supplier
- Rockland
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原理
- Mouse IgG Fc Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
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产品特性
- Anti-Mouse Serum antibody detects mouse serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Mouse Serum antibody is ideal for investigators involved in Cell Signaling, cellular biology and Signal Transduction research.
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.
Immunogen Type: Native Protein
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, WB, FACS, IF, ICC Polyclonal IgG DyLight 488
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, WB, FACS, IF, ICC Polyclonal IgG DyLight 488
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
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应用备注
- Application Note: Mouse IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
抗原
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