兔 anti-小鼠 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed
Quick Overview for 兔 anti-小鼠 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed (ABIN101848)
抗原
克隆类型
标记
应用范围
亚型
-
-
抗原表位
- F(ab')2 Region
-
适用
- 小鼠
-
宿主
- 兔
-
Supplier Product No.
- 610-4321
-
Supplier
- Rockland
-
原理
- Mouse IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed
-
交叉反应 （详细）
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against Mouse IgG F(c) or Human Serum Proteins.
-
产品特性
- Anti-Mouse IgG Peroxidase Antibody generated in rabbit detects reactivity to Mouse IgG.
-
纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
-
免疫原
- Optional[Immunogen]: Mouse IgG F(ab')2 fragment
-
-
Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
-
-
应用备注
- Application Note: Anti-Mouse IgG F(ab')2 Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:16,000 Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- IgG
-
物质类
- Antibody
-
背景
- Anti-Mouse IgG F(ab')2 Peroxidase Antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
-
