兔 anti-小鼠 IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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北京 101111
Quick Overview for 兔 anti-小鼠 IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN101845)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- F(ab')2 Region
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-4521
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Supplier
- Rockland
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原理
- Mouse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reactivity was observed against Mouse IgG F(c) or Human serum proteins.
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产品特性
- Anti-Mouse IgG Antibody generated in rabbit detects reactivity to Mouse IgG.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by solid phase adsorption to remove unwanted reactivities and extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Mouse IgG F(ab')2 fragment
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, WB, FACS, IF, ICC Polyclonal IgG DyLight 488
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, WB, FACS, IF, ICC Polyclonal IgG DyLight 488
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
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应用备注
- Application Note: This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,500 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Anti-Mouse IgG F(ab')2 Alkaline Phosphatase antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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