(2 references)
Quick Overview for 山羊 anti-猴 IgG Antibody (HRP) (ABIN101680)
抗原
IgG
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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适用
- 猴
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宿主
- 山羊
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Supplier Product No.
- 617-103-012
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Supplier
- Rockland
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原理
- Monkey IgG gamma Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgG and Monkey Serum.
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产品特性
- Anti-Monkey IgM (mu chain) peroxidase conjugated antibody generated in goat detects specifically Monkey IgM (mu chain).
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纯化方法
- MONKEY IgG gamma Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Monkey IgG (gamma chain) peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:1,000 - 1:85,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Tissue memory B cell repertoire analysis after ALVAC/AIDSVAX B/E gp120 immunization of rhesus macaques. ..." in: JCI insight, Vol. 1, Issue 20, pp. e88522, (2018) (PubMed).
: "Immunogenicity in mice and non-human primates of the Group A Streptococcal J8 peptide vaccine candidate conjugated to CRM197." in: Human vaccines & immunotherapeutics, Vol. 9, Issue 3, pp. 488-96, (2015) (PubMed).
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: "Tissue memory B cell repertoire analysis after ALVAC/AIDSVAX B/E gp120 immunization of rhesus macaques. ..." in: JCI insight, Vol. 1, Issue 20, pp. e88522, (2018) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- Anti-Monkey IgG (gamma chain) peroxidase conjugated antibody generated in goat detects specifically Monkey IgG (gamma chain). This secondary peroxidase conjugated antibody anti-Monkey is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
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