山羊 anti-人 IgG (Fc Region) Antibody (FITC)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 209-1203
- Supplier
- Rockland
- 原理
- Human IgG Fc Antibody Fluorescein Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
- 产品特性
- Anti-Human IgG (H&L) Fluorescein generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
- Optional[Immunogen]: Human IgG F(c) fragment
- 亚型
- IgG
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG2b PE
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Goat anti-Human IgG (Fc Region) antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgG (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS Polyclonal IgG Texas Red (TR)
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- 应用备注
- Application Note: Anti-Human IgG F(c) Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
- 浓度
- 10 mg/mL
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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