兔 anti-人 IgG (Heavy & Light Chain) Antibody (FITC)
(1 reference)
Quick Overview for 兔 anti-人 IgG (Heavy & Light Chain) Antibody (FITC) (ABIN101553)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 兔
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Supplier Product No.
- 209-4202
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Supplier
- Rockland
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原理
- Human IgG (H&L) Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgG and Human Serum.
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产品特性
- Anti-Human IgG F(c) Agarose Conjugated generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
- Optional[Immunogen]: Human IgG whole molecule
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Mouse anti-Human IgG (Fc Region) antibody
ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated
Mouse anti-Human IgG (Fc Region) antibody (FITC)FACS Monoclonal EM-07 IgG1 FITC
Mouse anti-Human IgG (Fc Region) antibodyAnimal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated
Mouse anti-Human IgG (Fab Region) antibodyELISA, WB, FACS Monoclonal 4A11 IgG1 kappa unconjugated
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG1 kappa PE
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
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应用备注
- Application Note: Anti-Human IgG (H&L) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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浓度
- 10 mg/mL
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Microbubble array diffusion assay for the detection of cell secreted factors." in: Lab on a chip, Vol. 14, Issue 18, pp. 3640-50, (2015) (PubMed).
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: "Microbubble array diffusion assay for the detection of cell secreted factors." in: Lab on a chip, Vol. 14, Issue 18, pp. 3640-50, (2015) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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