山羊 anti-人 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
Quick Overview for 山羊 anti-人 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN101535)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 山羊
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Supplier Product No.
- 609-102-123
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Supplier
- Rockland
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原理
- Human IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
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产品特性
- Anti-Human IgG F(c) Fluorescein Conjugated generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
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纯化方法
- Secondary Antibodies are prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Human IgG whole molecule
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Mouse anti-Human IgG (Fab Region) antibody (PE)
FACS Monoclonal 4A11 IgG1 kappa PE
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Mouse anti-Human IgG (Fc Region) antibody (Cy5)ELISA, FACS Monoclonal H2 IgG2b Cy5
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgG antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
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应用备注
- Application Note: Anti-Human IgG (H&L) Fluorescein Conjugated Antibody has been tested by dot blot. Secondary antibodies aid in the detection or sorting of target antigens by binding to the primary antibody. Anti-Human IgG (H&L) Fluorescein antibody is ideal for Flow Cytometry, Immunofluorescence Microscopy as well as other antibody detection methods where highly cross adsorbed (Pre Adsorbed) methods are required. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Secondary Antibody Conjugate at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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