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山羊 anti-人 IgG (Heavy & Light Chain) Antibody

ELISA, IHC, WB Polyclonal IgG unconjugated
Rockland
产品编号 ABIN101509
Supplier Product No.: 209-1102
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Heavy & Light Chain
    适用
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    山羊
    克隆类型
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    多克隆
    应用范围
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    209-1102
    Supplier
    Rockland
    原理
    Human IgG (H&L) Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum.
    产品特性
    Anti-Horse IgM antibody specifically detects horse IgM.
    纯化方法
    Anti-HUMAN IgG (H&L) (GOAT) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原
    Optional[Immunogen]: Human IgG whole molecule
    亚型
    IgG
  • 应用备注
    Application Note: Suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by researcher. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL
    浓度
    10 mg/mL
    缓冲液
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Bobo, Phelan, Rebhahn, Piepenbrink, Zheng, Mosmann, Kobie, DeLouise: "Microbubble array diffusion assay for the detection of cell secreted factors." in: Lab on a chip, Vol. 14, Issue 18, pp. 3640-50, (2015) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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