驴 anti-人 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
Quick Overview for 驴 anti-人 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN101476)
抗原
克隆类型
标记
应用范围
亚型
-
-
抗原表位
- Heavy & Light Chain
-
适用
- 人
-
宿主
- 驴
-
Supplier Product No.
- 609-705-002
-
Supplier
- Rockland
-
原理
- Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Human IgG and Human Serum.
-
产品特性
- Anti-Human IgG (H&L) Peroxidase generated in donkey detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.
-
纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
-
过滤
- Sterile filtered
-
免疫原
- Optional[Immunogen]: Human IgG whole molecule
-
-
Mouse anti-Human IgG (Fc Region) antibody
ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated
Mouse anti-Human IgG (Fc Region) antibodyAnimal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Mouse anti-Human IgG (Fab Region) antibodyELISA, WB, FACS Monoclonal 4A11 IgG1 kappa unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, WB Monoclonal EM-07 IgG1 HRP
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
-
-
应用备注
- Application Note: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate code # NPP-10) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
-
有效期
- 12 months
-
-
- IgG
-
物质类
- Antibody
-
背景
- It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
-
