山羊 anti-马 IgG (F(ab')2 Region) Antibody (HRP)
ELISA, IHC, WB
Polyclonal
IgG
HRP
1 image
(1 reference)
Quick Overview for 山羊 anti-马 IgG (F(ab')2 Region) Antibody (HRP) (ABIN101430)
抗原
IgG
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- F(ab')2 Region
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适用
- 马
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宿主
- 山羊
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Supplier Product No.
- 608-1304
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Supplier
- Rockland
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原理
- Horse IgG F(ab')2 Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum. No reaction was observed against Horse IgG F(c).
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产品特性
- Anti-Horse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Anti-Horse IgG was produced by repeated immunization with horse IgG F(ab')2 fragment in goat.
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Horse IgG F(ab')2 peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:50,000 - 1:150,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Pharmacokinetics of Snake Antivenom Following Intravenous and Intramuscular Administration in Envenomed Large Animal Model." in: Pharmaceutics, Vol. 17, Issue 2, (2025) (PubMed).
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: "Pharmacokinetics of Snake Antivenom Following Intravenous and Intramuscular Administration in Envenomed Large Animal Model." in: Pharmaceutics, Vol. 17, Issue 2, (2025) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- Anti-Horse F(ab')2 peroxidase conjugated antibody generated in goat detects specifically horse IgG F(ab')2. This secondary peroxidase conjugated antibody anti-Horse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
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