兔 anti-豚鼠 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB, DB
Polyclonal
IgG
Alkaline Phosphatase (AP)
1 image
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- 抗原 See all IgG products
- IgG
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抗原表位
- Fc Region
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适用
- 豚鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 606-4503
- Supplier
- Rockland
- 原理
- Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(ab').
- 产品特性
- Anti-Guinea Pig IgG Alkaline Phosphatase Antibody generated in rabbit detects guinea pig IgG.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
- Optional[Immunogen]: Guinea Pig IgG F(c) fragment
- 亚型
- IgG
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Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP
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- 应用备注
- Application Note: Anti-Guinea Pig IgG F(c) Alkaline Phosphatase conjugate has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:5,000 - 1:15,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Receptors bind the Fc portion of guinea pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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