Quick Overview for 山羊 anti-豚鼠 IgG (Fc Region) Antibody (ABIN101326)
抗原
IgG
克隆类型
多克隆
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- Fc Region
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适用
- 豚鼠
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宿主
- 山羊
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Supplier Product No.
- 606-1103
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Supplier
- Rockland
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原理
- Guinea Pig IgG Fc Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(ab).
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产品特性
- Anti-Guinea Pig IgG Antibody generated in goat detects guinea pig IgG.
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纯化方法
- Anti-Guinea Pig IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Guinea Pig IgG F(c) fragment
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Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
- Application Note: Anti-Guinea Pig IgG F(c) antibody has been assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-guinea Pig IgG F(c) Antibody. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Receptors bind the Fc portion of Guinea Pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Guinea Pig IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
抗原
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