山羊 anti-豚鼠 IgG (F(ab')2 Region) Antibody
ELISA, IHC, WB
Polyclonal
unconjugated
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- 抗原 See all IgG products
- IgG
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抗原表位
- F(ab')2 Region
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适用
- 豚鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 106-1104
- Supplier
- Rockland
- 原理
- Guinea Pig IgG F(ab')2 Antibody
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, guinea pig IgG, guinea pig serum, Guinea Pig IgG F(ab')2. No reaction was observed against Guinea Pig IgG F(c).
- 产品特性
- Anti-Guinea Pig IgG Antibody generated in goat detects guinea pig IgG.
- 纯化方法
- This product was prepared from monospecific antiserum by delipidation and defibrination.
- 免疫原
- Optional[Immunogen]: Guinea Pig IgG F(ab')2 fragment
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Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP
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- 应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
- 浓度
- 90 mg/mL
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Anti-Guinea Pig IgG F(ab')2 Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Guinea Pig IgG F(c) will not bind Guinea Pig IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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