(1 reference)
Quick Overview for 兔 anti-山羊 IgG (Fc Region) Antibody (HRP) (ABIN101255)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Fc Region
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适用
- 山羊
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宿主
- 兔
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Supplier Product No.
- 605-4303
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Supplier
- Rockland
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原理
- Goat IgG Fc Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab) .
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产品特性
- Anti-Goat IgG Peroxidase Antibody generated in rabbit detects goat IgG.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
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免疫原
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Immunogen: Anti-Goat IgG F(c) was produced by repeated immunization with Goat IgG F(c) fragment in rabbit.
Immunogen Type: Native Protein
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Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550
Pig anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Pig anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Guinea Pig anti-Goat IgG antibodyELISA, WB Polyclonal IgG unconjugated
Chicken anti-Goat IgG antibody (DyLight 488)WB, IF Polyclonal IgY DyLight 488
Pig anti-Goat IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedELISA Polyclonal IgG PE
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应用备注
- Application Note: Goat IgG F(c) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 μg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:30,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Downregulation of human epidermal growth factor receptor 2 by short hairpin RNA increases chemosensitivity of human ovarian cancer cells." in: Oncology letters, Vol. 9, Issue 5, pp. 2211-2217, (2015) (PubMed).
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: "Downregulation of human epidermal growth factor receptor 2 by short hairpin RNA increases chemosensitivity of human ovarian cancer cells." in: Oncology letters, Vol. 9, Issue 5, pp. 2211-2217, (2015) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- Anti-Goat IgG F(ab')2 Peroxidase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.
抗原
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