兔 anti-山羊 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))
Quick Overview for 兔 anti-山羊 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) (ABIN101250)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Fc Region
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适用
- 山羊
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宿主
- 兔
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Supplier Product No.
- 605-4503
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Supplier
- Rockland
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原理
- Goat IgG Fc Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab).
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产品特性
- Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Goat IgG F(c) fragment
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Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550
Mouse anti-Goat IgG antibodyAnimal-Free ELISA Monoclonal AB326-1-B10 IgG2a Recombinant Antibody unconjugated
Mouse anti-Goat IgG antibodyAnimal-Free ELISA Monoclonal AB326-1-C04 IgG2a Recombinant Antibody unconjugated
Pig anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, FLISA Polyclonal IgG HRP
Donkey anti-Goat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
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应用备注
- Application Note: Anti-Goat IgG F(c) Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:4,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Receptors bind the Fc portion of goat IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
抗原
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