ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Supplier Product No.
105-4103
Supplier
Rockland
原理
Goat IgG Fc Antibody
交叉反应 (详细)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab).
产品特性
Anti-Goat IgG Antibody generated in rabbit detects goat IgG.
纯化方法
This product was prepared from monospecific antiserum by a delipidation and defibrination.
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
浓度
80 mg/mL
缓冲液
Optional[Buffer]: None, Preservative:None
储存液
Without preservative
储存条件
4 °C,-20 °C
储存方法
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Receptors bind the Fc portion of goat IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.