驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
(3 references)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 山羊
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 605-706-125
- Supplier
- Rockland
- 原理
- Goat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Horse, Hamster, Mouse, Rabbit and Rat Serum Proteins.
- 产品特性
- Anti-Goat IgG Biotin Antibody generated in donkey detects goat IgG.
- 纯化方法
- Anti-Goat IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
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Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.
Immunogen Type: Native Protein
- 亚型
- IgG
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Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550
Pig anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Guinea Pig anti-Goat IgG antibodyELISA, WB Polyclonal IgG unconjugated
Chicken anti-Goat IgG antibody (DyLight 488)WB, IF Polyclonal IgY DyLight 488
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Pig anti-Goat IgG (Heavy & Light Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
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- 应用备注
- Application Note: Goat IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:60,000 ELISA Dilution: 1:600,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Skn-1a/Pou2f3 functions as a master regulator to generate Trpm5-expressing chemosensory cells in mice." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189340, (2018) (PubMed).
: "MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6." in: Experimental and therapeutic medicine, Vol. 12, Issue 6, pp. 4029-4034, (2016) (PubMed).
: "Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring." in: Biology of reproduction, Vol. 94, Issue 4, pp. 94, (2016) (PubMed).
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: "Skn-1a/Pou2f3 functions as a master regulator to generate Trpm5-expressing chemosensory cells in mice." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189340, (2018) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Goat IgG (H&L) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology research.
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