驴 anti-山羊 IgG (Heavy & Light Chain) Antibody - Preadsorbed
(1 reference)
Quick Overview for 驴 anti-山羊 IgG (Heavy & Light Chain) Antibody - Preadsorbed (ABIN101178)
抗原
克隆类型
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 山羊
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宿主
- 驴
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Supplier Product No.
- 605-701-125
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Supplier
- Rockland
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原理
- Goat IgG (H&L) Antibody Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins. Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above.
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产品特性
- Anti-Goat IgG Antibody generated in donkey detects goat IgG.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Goat IgG whole molecule
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Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550
Mouse anti-Goat IgG antibodyAnimal-Free ELISA Monoclonal AB326-1-B10 IgG2a Recombinant Antibody unconjugated
Mouse anti-Goat IgG antibodyAnimal-Free ELISA Monoclonal AB326-1-C04 IgG2a Recombinant Antibody unconjugated
Pig anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, FLISA Polyclonal IgG HRP
Donkey anti-Goat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
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应用备注
- Application Note: Anti-Goat IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:150,000 - 1:250,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.25 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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: "Single-molecule pull-down for studying protein interactions." in: Nature protocols, Vol. 7, Issue 3, pp. 445-52, (2012) (PubMed).
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: "Single-molecule pull-down for studying protein interactions." in: Nature protocols, Vol. 7, Issue 3, pp. 445-52, (2012) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
抗原
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