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驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (HRP)

ELISA, IHC, WB Polyclonal IgG HRP

ELISA results of purified Donkey anti-Goat IgG antibody Peroxidase conjugated tested against purified Goat IgG. (驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (HRP))

1 image

(1 reference)

产品编号 ABIN101172

Supplier Product No.: 605-703-002

发货至: 中国

Datasheet as PDF

Quick Overview for 驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN101172)

抗原

IgG

克隆类型

多克隆

标记

HRP

应用范围

ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

亚型

IgG

抗原表位
2396

517

417

146

130

128

66

29

28

28

21

9

5

5

2

2

2

2

2

1

1

1
Heavy & Light Chain
适用
752

703

607

468

409

244

213

176

146

141

124

110

107

101

82

62

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
山羊
宿主
1773

1583

566

282

122

112

41

32

22

21

15

11

11
驴

Supplier Product No.

605-703-002

Supplier

Rockland

原理

Goat IgG (H&L) Antibody Peroxidase Conjugated

交叉反应（详细）

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum.

产品特性

Anti-Goat IgG Fluorescein Antibody generated in donkey detects goat IgG.

纯化方法

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.

免疫原

Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with Goat IgG whole molecule in donkey.
Immunogen Type: Native Protein
Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550

Mouse anti-Goat IgG antibody
Animal-Free ELISA Monoclonal AB326-1-B10 IgG2a Recombinant Antibody unconjugated

Mouse anti-Goat IgG antibody
Animal-Free ELISA Monoclonal AB326-1-C04 IgG2a Recombinant Antibody unconjugated

Pig anti-Goat IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Rabbit anti-Goat IgG (Whole Molecule) antibody (HRP)
ELISA, WB, DB Polyclonal IgG HRP

Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550

Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 532

Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)

Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, FLISA Polyclonal IgG HRP

Donkey anti-Goat IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

应用备注

Application Note: Anti-Goat IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. It is also suitable in IHC. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:150,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

2.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

储存液

Gentamicin sulfate

注意事项

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Gauthamadasa, Vaitinadin, Dressman, Macha, Homan, Greis, Silva: "Apolipoprotein A-II-mediated conformational changes of apolipoprotein A-I in discoidal high density lipoproteins." in: The Journal of biological chemistry, Vol. 287, Issue 10, pp. 7615-25, (2012) (PubMed).
抗原

IgG

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

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