山羊 anti-驴 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB, DB
Polyclonal
IgG
Alkaline Phosphatase (AP)
Quick Overview for 山羊 anti-驴 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN101124)
抗原
IgG
克隆类型
多克隆
标记
Alkaline Phosphatase (AP)
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- 驴
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宿主
- 山羊
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Supplier Product No.
- 616-1502
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Supplier
- Rockland
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原理
- Donkey IgG (H&L) Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Donkey IgG, and Donkey Serum.
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产品特性
- Anti-Donkey IgG Peroxidase Antibody generated in goat detects donkey IgG.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Donkey IgG whole molecule
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应用备注
- Application Note: Anti-Donkey IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
抗原
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