山羊 anti-Cow IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB
Polyclonal
IgG
Alkaline Phosphatase (AP)
Quick Overview for 山羊 anti-Cow IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP)) (ABIN100912)
抗原
IgG
克隆类型
多克隆
标记
Alkaline Phosphatase (AP)
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- F(ab')2 Region
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适用
- Cow
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宿主
- 山羊
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Supplier Product No.
- 601-1504
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Supplier
- Rockland
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原理
- Bovine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Bovine IgG, Bovine IgG F(ab')2 and Bovine Serum. No reaction was observed against Bovine IgG F(c).
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Bovine IgG F(ab')2 fragment
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应用备注
- Application Note: Anti-Bovine IgG F(ab')2 Alkaline Phosphatase Conjugate has been tested by ELISA and is assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
, Preservative:0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
抗原
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