兔 anti-Cow IgG (Heavy & Light Chain) Antibody
ELISA, IHC, WB
Polyclonal
unconjugated
Quick Overview for 兔 anti-Cow IgG (Heavy & Light Chain) Antibody (ABIN100896)
抗原
IgG
克隆类型
多克隆
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
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抗原表位
- Heavy & Light Chain
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适用
- Cow
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宿主
- 兔
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Supplier Product No.
- 101-4102
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Supplier
- Rockland
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原理
- Bovine IgG (H&L) Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Bovine IgG and Bovine Serum.
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产品特性
- Anti-Bovine serum antibody detects bovine serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Bovine serum antibody is ideal for investigators involved in Cell Signaling, cellular biology and Signal Transduction research.
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Optional[Immunogen]: Bovine IgG whole molecule
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应用备注
- Application Note: Anti-Bovine IgG antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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浓度
- 75.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
抗原
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