山羊 anti-Cow IgG (Heavy & Light Chain) Antibody
ELISA, IHC, WB, DB
Polyclonal
IgG
unconjugated
1 image
Quick Overview for 山羊 anti-Cow IgG (Heavy & Light Chain) Antibody (ABIN100887)
抗原
IgG
克隆类型
多克隆
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- Cow
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宿主
- 山羊
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Supplier Product No.
- 201-1102
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Supplier
- Rockland
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原理
- Bovine IgG (H&L) Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG and Bovine Serum.
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产品特性
- Sodium/potassium-transporting ATPase subunit alpha-1 is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
- Optional[Immunogen]: Bovine IgG whole molecule
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应用备注
- Application Note: Anti-Bovine IgG Secondary Antibodies have been tested by dot blot and are designed for Western Blotting, ELISA and Immunohistochemistry. Secondary antibodies can also be used for a variety of other applications such as Assay Development, Flow Cytometry and in situ hybridization. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
抗原
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