山羊 anti-小鼠 IgA, IgG, IgM Antibody (Texas Red (TR))
FACS, WB, DB, FM, FLISA
Polyclonal
IgG
Texas Red (TR)
1 image
Quick Overview for 山羊 anti-小鼠 IgA, IgG, IgM Antibody (Texas Red (TR)) (ABIN100808)
抗原
IgA, IgG, IgM
克隆类型
多克隆
标记
Texas Red (TR)
应用范围
Flow Cytometry (FACS), Western Blotting (WB), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA
亚型
IgG
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 610-109-130
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Supplier
- Rockland
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原理
- Mouse IgG IgA IgM (H&L) Antibody Texas Red™ Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
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产品特性
- Anti-Mouse IgG Texas Red Antibody generated in goat detects reactivity to Mouse IgG.
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纯化方法
- Mouse IgG IgA IgM (H&L) Antibody Texas Red™ Conjugated was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
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应用备注
- Application Note: Mouse IgG IgA IgM (H&L) Antibody Texas Red™ Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgA, IgG, IgM
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别名
- IgG,IgA,IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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