山羊 anti-人 IgA Antibody (HRP)
Quick Overview for 山羊 anti-人 IgA Antibody (HRP) (ABIN100719)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 人
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宿主
- 山羊
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Supplier Product No.
- 609-1306
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Supplier
- Rockland
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原理
- Human IgA alpha Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgA and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes.
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产品特性
- Anti-Human IgG F(ab')2 Peroxidase Antibody generated in goat recognizes the dimeric Fab portion of the human IgG molecule. Human IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind human IgG F(c) will not bind human IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Anti-Human IgA alpha heavy chain was produced by repeated immunization with Human IgA alpha heavy chain in goat.
Immunogen Type: Native Protein
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Rabbit anti-Human IgA antibody
IHC Monoclonal MSVA-700R IgG Recombinant Antibody unconjugated
Mouse anti-Human IgA (Heavy Chain) antibodyELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
Goat anti-Human IgA antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgA antibody (HRP) - PreadsorbedELISA Polyclonal IgG HRP
Goat anti-Human IgA antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (Biotin)ELISA, WB, FACS Polyclonal IgG Biotin
Goat anti-Human IgA (Heavy Chain) antibody (FITC)ELISA, FACS Polyclonal IgG FITC
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应用备注
- Application Note: Anti-Human IgA Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:200,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgA
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物质类
- Antibody
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背景
- Anti-Human IgA Peroxidase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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