Sar1 抗体
(3 references)Our Local Distributor
北京 101111
Quick Overview for Sar1 抗体 (ABIN7477852)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-Sar1 | Secretion-associated and Ras-related protein 1
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交叉反应 （详细）
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Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Petunia hybrida, Triticum aestivum, Zea mays
Not reactive in: Nicotiana tabacum -
预测反应
- Citrus clementina,Medicago truncatula,Brassica rapa,Hordeum vulgare,Solanum tuberosum,Solanum lycopersicum,Populus trichocarpa,Glycine max,Vitis vinifera,Capsella rubella,Oryza sativa,Sorghum bicolor,Brachypodium distachyon,Brassica campestris,Brassica napus,Theobroma cacao,Triticum aestivum,Ricinus communis
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纯化方法
- Serum
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免疫原
- GST fusion of full length recombinant Sar1 of Arabidopsis thaliana UniProt: Q8VYP7
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应用备注
- 1 : 50 (IG), 1 : 500 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).
: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).
: "Protein composition analysis of oil bodies from maize embryos during germination." in: Journal of plant physiology, Vol. 168, Issue 5, pp. 510-3, (2011) (PubMed).
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: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).
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- Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))
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背景
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Background: Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins - Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.
Additional information: For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation.
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分子量
- 21 kDa (Arabidopsis thaliana)
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基因ID
- 825430
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UniProt
- Q8VYP7
抗原
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