RGL2 抗体
This 兔 多克隆 antibody specifically detects RGL2 in WB. It exhibits reactivity toward Arabidopsis thaliana.
产品编号 ABIN4966294
发货至:
中国
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Quick Overview for RGL2 抗体 (ABIN4966294)
抗原
RGL2
(DELLA Protein RGL2 (RGL2))
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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交叉反应 （详细）
- Not reactive in: Triticum sp.
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免疫原
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450
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应用备注
- 1 : 1000 with standard ECL (WB) on transfected Arabidopsis thaliana protoplasts
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说明
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As this protein is expressed in tissue in very low levels, please load at least 50 μg/well.Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 μl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water.
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储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- RGL2 (DELLA Protein RGL2 (RGL2))
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别名
- RGA-like protein 2 (DELLA protein) (RGL2)
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背景
- DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.
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分子量
- 60 kDa
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UniProt
- Q8GXW1
抗原
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