VEGF 蛋白
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北京 101111
Quick Overview for VEGF 蛋白 (ABIN988283)
抗原
See all VEGF 蛋白生物活性
宿主
资源
纯度
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产品特性
- Resuspend cells in assay media (Earle Salts 199X Gibco #11150, without h-EGF) containing 10 % HI-FBS and transfer 100 ul/well to assay plate (5,100 cells/well passage#2). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml/well to cells in assay plate. Final assay volume is 200 ml/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng/ml.
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内毒素水平
- level is less than 0.1 ng per myg (1 EU/myg) of VEGF
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Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein
HPLC verified VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein (Biotin)VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (Active) proteinVEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Vascular Endothelial Growth Factor (VEGF) (Active) proteinVEGF 宿主: 人 宿主: Yeast (Pichia pastoris) > 95 % by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein (His tag)VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-190) (Active) proteinHPLC verified VEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-147) (Active) protein (AVI tag,His tag,Biotin)VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (Active) proteinVEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-147) (Active) protein (His tag)VEGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Vascular Endothelial Growth Factor (VEGF) (AA 27-146) (Active) proteinVEGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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别名
- Vascular Endothelial Growth Factor
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背景
- VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Synonym: VEGF 6. Formulation: Lyophilized from 25 mM HEPES and 150 mM NaCl, pH 7.0.
抗原
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