PreScission Protease (PSP) protein (GST tag)
宿主: Enterovirus (EV71), Schistosoma japonicum
宿主: 大肠杆菌（E. Coli）
Recombinant
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- 抗原
- PreScission Protease (PSP)
- 蛋白类型
- Recombinant
- 宿主
- Enterovirus (EV71), Schistosoma japonicum
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资源
- 大肠杆菌（E. Coli）
- 标记
- GST tag
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- 应用备注
- During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.
- 限制
- 仅限研究用
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- 缓冲液
- Cleavage Buffer: 50mM Tris-HCl, pH7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5°C prior to use.
- 储存条件
- -20 °C
- 储存方法
- Should be stored in small aliquots at -20°C to -80°C for long term.
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- 抗原
- PreScission Protease (PSP)
- 物质类
- Viral Protein
- 背景
- PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core AA sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well. Synonym: PreScission Protease(PSP). .
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