IFN alpha 2b 蛋白
This Recombinant IFN alpha 2b protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 1 publication.
(1 reference)Quick Overview for IFN alpha 2b 蛋白 (ABIN987980)
抗原
IFN alpha 2b
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95.0 % as determined by:(a) Analysis by RP-HPLC.(b) Anion-exchange FPLC.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
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序列
- Cys24-Glu188 (Lys46Arg)
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产品特性
- ED50 < 0.05 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 2.0 × 10^7 U/mg.
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内毒素水平
- < 0.2 EU/μg of protein by gel clotting method
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IFN alpha 2b (AA 24-188) (Active) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended to reconstitute the lyophilized Recombinant Human Interferon alpha 2b (IFN alpha2b) in sterile 18M-omega-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of IFN-alpha 2b as a Reference Standard.
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储存条件
- -20 °C
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: "Response of hepatitis C virus to long-term passage in the presence of alpha interferon: multiple mutations and a common phenotype." in: Journal of virology, Vol. 87, Issue 13, pp. 7593-607, (2013) (PubMed).
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: "Response of hepatitis C virus to long-term passage in the presence of alpha interferon: multiple mutations and a common phenotype." in: Journal of virology, Vol. 87, Issue 13, pp. 7593-607, (2013) (PubMed).
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- IFN alpha 2b
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别名
- IFN-alpha 2b
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背景
- Human Interferon alpha 2b (IFN alpha2b) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Da. The Interferon alpha 2b (IFN alpha2b) gene was obtained from human leukocytes. The Recombinant Human Interferon alpha 2b (IFN alpha2b) is purified by proprietary chromatographic techniques. Synonym: rHuIFN-alpha-2b, rHuIFNalpha-2b, rHuIFN-a-2b, rHuIFN-alfa-2b, rHuIFNalfa-2b, Interferon-alpha-2b, Interferonalpha-2b, Interferon-a-2b, Interferon-alfa-2b, Interferonalfa-2. Formulation: Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer.
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分子量
- ~19.2 kDa, on SDS-PAGE under reducing conditions.
抗原
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