GM-CSF 蛋白
Quick Overview for GM-CSF 蛋白 (ABIN987967)
抗原
See all GM-CSF (CSF2) 蛋白生物活性
宿主
资源
纯度
-
-
产品特性
- Resuspend cells in assay media free of h-GM-CSF containing 10 % FBS and 2X Gentamicin. Transfer 100ml culture/well to assay plate (19,500 cells/well, passage # 13). Serial dilute h-GM-CSF in assay media in duplicate. Transfer 100 ml/well serial diluted h-GM-CSF to cells in assay plate. Final assay volume of each well is 200 ml containing 2X Gentamicin and h-GM-CSF. Incubate 70 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well. Incubate 37°C and read OD at 490 nm. The ED50 for this effect is 0.06 - 0.09 ng/ml.
-
内毒素水平
- level is less than 0.03 ng per myg (0.3 EU/myg) of human GMCSF
-
-
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein
CSF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)CSF2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 宿主: 人 宿主: HEK-293 Cells > 95 % AcA, CC Active
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
储存条件
- -20 °C
-
-
- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
-
别名
- Granulocyte Macrophage Colony Stimulating Factor
-
背景
- GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Synonym: rHuGM-CSF 7, hGM-CS. Formulation: Lyophilized from a 0.2 mym filtered solution in 10 mM PB, pH7.0.
-
途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
-
