Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependant stimulation of the proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to aof 1.0×107 IU/mg
纯度
> 98 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rRhGM-CSF as determined by LAL method
CSF2
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at
GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. Synonym: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Rhesus Macaque. Formulation: Lyophilized from a 0.2µm filtered concentratedcsolution in PBS, pH 7.4.
分子量
Approximately 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.