CSF3
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 98 % as determined by:(a) Analysis by SEC-HPLC(b)Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
Active
analysis The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser-Phe-Leu-Leu. p to grams level.
产品特性
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to aof 6.0×107 IU/mg
纯度
> 98 % as determined by:(a) Analysis by SEC-HPLC(b)Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
内毒素水平
Level Less than 0.03ng/µg (0.3 IEU/µg) determined by LAL test
Crystallography grade
CSF3
宿主: 小鼠
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
It is recommended to reconstitute the lyophilized Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) in sterile 18 M-omega-cm H2O not less than 100 myg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.
储存条件
-20 °C
Xie, Gorodetsky, Micewicz, Micevicz, Mackenzie, Gaberman, Levdansky, McBride: "Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits." in: The Journal of investigative dermatology, Vol. 133, Issue 2, pp. 553-61, (2013) (PubMed).
Human Granulocyte Colony Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da. Synonym: rHuG-CSF, G-CS. Formulation: The protein was lyophilized after extensive dialysis against 10mM HAc-NaAc, pH4.0, 150mM NaCl, 0.004% Tween 80, 5% Mannitol buffer.
分子量
18,800 Da Isoelectric Point The main zone between 5.8-6.6 analysis by IEF UV Scan The maximal absorption wave is 278+/-3 nm.