电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

G-CSF 蛋白

CSF3 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 98 % as determined by:(a) Analysis by SEC-HPLC(b)Analysis by reducing and non-reducing SDS-PAGE silver-stained gel Active
产品编号 ABIN987880
发货至: 中国
  • 抗原 See all G-CSF (CSF3) 蛋白
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    蛋白类型
    Recombinant
    生物活性
    Active
    宿主
    • 28
    • 9
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    资源
    • 23
    • 11
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    大肠杆菌(E. Coli)
    序列
    analysis The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser-Phe-Leu-Leu. p to grams level.
    产品特性
    The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to aof 6.0×107 IU/mg
    纯度
    > 98 % as determined by:(a) Analysis by SEC-HPLC(b)Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
    内毒素水平
    Level Less than 0.03ng/µg (0.3 IEU/µg) determined by LAL test
    Top Product
    Discover our top product CSF3 蛋白
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is recommended to reconstitute the lyophilized Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) in sterile 18 M-omega-cm H2O not less than 100 myg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.
    储存条件
    -20 °C
  • Xie, Gorodetsky, Micewicz, Micevicz, Mackenzie, Gaberman, Levdansky, McBride: "Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits." in: The Journal of investigative dermatology, Vol. 133, Issue 2, pp. 553-61, (2013) (PubMed).

  • 抗原
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    别名
    Granulocyte Colony Stimulating Factor (G-CSF) (CSF3 产品)
    别名
    CSF3 Protein, G-CSF Protein, gcsf Protein, Csfg Protein, MGI-IG Protein, C17orf33 Protein, CSF3OS Protein, GCSF Protein, Gcsf Protein, colony stimulating factor 3 Protein, colony stimulating factor 3 (granulocyte) a Protein, colony stimulating factor 3 (granulocyte) Protein, CSF3 Protein, csf3a Protein, Csf3 Protein
    背景
    Human Granulocyte Colony Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da. Synonym: rHuG-CSF, G-CS. Formulation: The protein was lyophilized after extensive dialysis against 10mM HAc-NaAc, pH4.0, 150mM NaCl, 0.004% Tween 80, 5% Mannitol buffer.
    分子量
    18,800 Da Isoelectric Point The main zone between 5.8-6.6 analysis by IEF UV Scan The maximal absorption wave is 278+/-3 nm.
    途径
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
You are here:
客服