G-CSF 蛋白
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北京 101111
Quick Overview for G-CSF 蛋白 (ABIN987880)
抗原
See all G-CSF (CSF3) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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序列
- analysis The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser-Phe-Leu-Leu. p to grams level.
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产品特性
- The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to aof 6.0×107 IU/mg
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内毒素水平
- Level Less than 0.03ng/µg (0.3 IEU/µg) determined by LAL test
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Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) protein
CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (Active) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-208) (Active) proteinCSF3 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) protein (His tag)CSF3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 22-214) protein (His-IF2DI Tag)CSF3 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) protein (GST tag)CSF3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, AcA Active
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) proteinCSF3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 31-204) (Active) proteinCSF3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended to reconstitute the lyophilized Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) in sterile 18 M-omega-cm H2O not less than 100 myg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.
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储存条件
- -20 °C
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: "Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits." in: The Journal of investigative dermatology, Vol. 133, Issue 2, pp. 553-61, (2013) (PubMed).
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: "Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits." in: The Journal of investigative dermatology, Vol. 133, Issue 2, pp. 553-61, (2013) (PubMed).
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- Granulocyte Colony Stimulating Factor (G-CSF)
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背景
- Human Granulocyte Colony Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da. Synonym: rHuG-CSF, G-CS. Formulation: The protein was lyophilized after extensive dialysis against 10mM HAc-NaAc, pH4.0, 150mM NaCl, 0.004% Tween 80, 5% Mannitol buffer.
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分子量
- 18,800 Da Isoelectric Point The main zone between 5.8-6.6 analysis by IEF UV Scan The maximal absorption wave is 278+/-3 nm.
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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