Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using murine lymphocytes is less than 1000 ng/ml, corresponding to a specific activity of >, 1.0 × 103 IU/mg.
纯度
> 98 % by SDS-PAGE and HPLC analyses.
内毒素水平
Level Less than 1EU/µg of rMuCXCL16 as determined by LAL method
CXCL16
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
>95 % as determined by SDS-PAGE
Func
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
Mouse CXCL16 (CXC chemokine 16) is a nonELR motifcontaining CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine and CXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa residue signal peptide,an 88 aa residue chemokine domain, an 87 aa residue mucinlike spacer region, a 22 aa residue transmembrane domain, and a 23 aa residue cytoplasmic tail. Mouse and human CXCL16 share 49% overall aa identity and 70% similarity in the chemokine domains. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and by cells in the splenic red pulp both as membranebound and soluble forms. Based on northern blot analysis,CXCL16 is also expressed in some nonlymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6/Bonzo (STRL33 and TYMSTR), a receptor previously shown to be a coreceptor for HIV entry.CXCR6 is expressed on naive CD8 cells, naturalkiller T cells and activated CD8 and CD4 T cells. Synonym: CXCL16, Mouse. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS.
分子量
Recombinant murine CXCL16 is a 9.9 kDa protein containing 88 amino acid residues.