Streptavidin Protein (Alkaline Phosphatase (AP))
Quick Overview for Streptavidin Protein (Alkaline Phosphatase (AP)) (ABIN964538)
抗原
See all Streptavidin 蛋白蛋白类型
宿主
资源
应用范围
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标记
- This Streptavidin protein is labelled with Alkaline Phosphatase (AP).
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Supplier Product No.
- s000-05
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Supplier
- Rockland
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原理
- Streptavidin Alkaline Phosphatase Conjugated
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纯化方法
- Streptavidin-Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin. Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf inte
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过滤
- Sterile filtered
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Streptavidin protein (HRP)
宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, ELISA, FM, IHC, DB, IF, IEM
Streptavidin protein (PE)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native ELISA, FM, FACS, MA, DB, IF, BCA, Func
Streptavidin (AA 25-183) protein (His tag)宿主: Streptomyces 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Streptavidin (AA 37-159) protein (His tag)宿主: Streptomyces 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Streptavidin protein (TRITC)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native FM, FLISA, IHC, FISH
Streptavidin protein (Atto 425)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, FM, FLISA, DB, IF
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Streptavidin Alkaline Phosphatase conjugated has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment
Western Blot Dilution: 1:1,000 - 1:4,000
ELISA Dilution: 1:8,000 - 1:32,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE. Dilute only prior to immediate use.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss o Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Streptavidin
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背景
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Synonyms: SA alkaline phosphatase conjugate, S avidin conjugated to alkaline phosphatase, alkaline phosphatase conjugated to streptococcus avidin, streptavidin Alk Phos, SA-ALP, ALP conjugated streptavidin
Background: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditio
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UniProt
- P22629
抗原
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