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Streptavidin Protein (Alkaline Phosphatase (AP))

This Native Streptavidin protein is expressed in Streptomyces avidinii.
Rockland
产品编号 ABIN964538
Supplier Product No.: s000-05
发货至: 中国
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Quick Overview for Streptavidin Protein (Alkaline Phosphatase (AP)) (ABIN964538)

抗原

See all Streptavidin 蛋白
Streptavidin

蛋白类型

Native

宿主

  • 62
  • 2
  • 2
  • 1
  • 1
Streptomyces avidinii

资源

  • 34
  • 12
  • 6
  • 2
  • 1
Streptomyces avidinii

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • 标记

    This Streptavidin protein is labelled with Alkaline Phosphatase (AP).

    Supplier Product No.

    s000-05

    Supplier

    Rockland

    原理

    Streptavidin Alkaline Phosphatase Conjugated

    纯化方法

    Streptavidin-Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin.   Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf inte

    过滤

    Sterile filtered
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  • 应用备注

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Streptavidin Alkaline Phosphatase conjugated has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment

    Western Blot Dilution: 1:1,000 - 1:4,000

    ELISA Dilution: 1:8,000 - 1:32,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    DO NOT FREEZE. Dilute only prior to immediate use.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss o Expiration date is one (1) year from date of receipt.

    有效期

    12 months
  • 抗原

    Streptavidin

    背景

    Synonyms: SA alkaline phosphatase conjugate, S avidin conjugated to alkaline phosphatase, alkaline phosphatase conjugated to streptococcus avidin, streptavidin Alk Phos, SA-ALP, ALP conjugated streptavidin

    Background: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditio

    UniProt

    P22629
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