Streptavidin Protein (HRP)
(4 references)
Quick Overview for Streptavidin Protein (HRP) (ABIN964537)
抗原
See all Streptavidin 蛋白蛋白类型
宿主
资源
应用范围
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标记
- This Streptavidin protein is labelled with HRP.
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Supplier Product No.
- s000-03
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Supplier
- Rockland
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原理
- Streptavidin Peroxidase Conjugated
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纯化方法
- Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
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Streptavidin protein (PE)
宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native ELISA, FACS, MA, BCA, DB, IF, Func, FM
Streptavidin (AA 37-159) protein (His tag)宿主: Streptomyces 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Streptavidin protein (TRITC)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native IHC, FM, FISH, FLISA
Streptavidin protein (Atto 425)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, DB, IF, FM, FLISA
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Streptavidin Peroxidase has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Bl
Western Blot Dilution: 1:10,000 - 1:40,000
ELISA Dilution: 1:20,000 - 1:200,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent) (in 1.0 mL)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." in: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).
: "A complement-microglial axis drives synapse loss during virus-induced memory impairment." in: Nature, Vol. 534, Issue 7608, pp. 538-43, (2016) (PubMed).
: "TREM2 regulates microglial cell activation in response to demyelination in vivo." in: Acta neuropathologica, Vol. 129, Issue 3, pp. 429-47, (2015) (PubMed).
: "The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli." in: Molecular microbiology, Vol. 79, Issue 5, pp. 1168-81, (2011) (PubMed).
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: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." in: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).
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- Streptavidin
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背景
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Synonyms: HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin
Background: Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, im
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UniProt
- P22629
抗原
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