Biotin Protein (beta-Gal)
Quick Overview for Biotin Protein (beta-Gal) (ABIN964127)
抗原
宿主
资源
应用范围
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标记
- This Biotin protein is labelled with beta-Gal.
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Supplier Product No.
- b000-17
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Supplier
- Rockland
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原理
- Biotin Beta Galactosidase Conjugated
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纯化方法
- Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin. Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and an
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过滤
- Sterile filtered
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Biotin protein (Alkaline Phosphatase (AP))
宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
Biotin protein (Glucose Oxidase)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB, MA
Biotin protein (HRP)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Biotin Beta Galactosidase Conjugated has been tested by ELISA and dot blot and is assayed against 1.0 μg of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A wor
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:8,000 - 1:32,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- 0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol, pH 7.8
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注意事项
- DO NOT FREEZE. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
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Synonyms: Vitamin H, Coenzyme R, Vitamin B7, β-galactosidase, β-gal, beta-gal, Biotin Beta Galactosidase Conjugated, Biotin Beta Gal Conjugated
Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav
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