Quick Overview for Biotin Protein (Alkaline Phosphatase (AP)) (ABIN964125)
抗原
宿主
资源
应用范围
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标记
- This Biotin protein is labelled with Alkaline Phosphatase (AP).
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Supplier Product No.
- b000-05
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Supplier
- Rockland
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原理
- Biotin Alkaline Phosphatase Conjugated
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纯化方法
- This product was prepared from electrophoretically pure Alkaline Phosphatase and Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.
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过滤
- Sterile filtered
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Biotin protein (Glucose Oxidase)
宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB, MA
Biotin protein (HRP)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
Biotin protein (beta-Gal)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Biotin Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:8,000 - 1:32,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
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Synonyms: Biotin Alkaline Phosphatase Conjugated, Biotin alk phos Conjugated, alkaline phosphatase conjugated biotin
Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav
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