Quick Overview for Biotin Protein (HRP) (ABIN964124)
抗原
宿主
资源
应用范围
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标记
- This Biotin protein is labelled with HRP.
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Supplier Product No.
- b000-03
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Supplier
- Rockland
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原理
- Biotin Peroxidase Conjugated
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纯化方法
- Biotin Peroxidase Conjugated was prepared from chromatographically purified biotin. Biotin Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Biotin.
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Biotin protein (Alkaline Phosphatase (AP))
宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
Biotin protein (Glucose Oxidase)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB, MA
Biotin protein (beta-Gal)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Biotin Peroxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
Western Blot Dilution: 1:10,000 - 1:40,000
ELISA Dilution: 1:20,000 - 1:200,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent) (in 5.0 mL)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Biotin Peroxidase Conju Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
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Synonyms: Vitamin H, Coenzyme R, Vitamin B7, Biotin Peroxidase Conjugated, Biotin HRP Conjugated
Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav
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