Thioredoxin Reductase Protein (TrxR) (AA 1-321)
This Recombinant Thioredoxin Reductase protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for Thioredoxin Reductase Protein (TrxR) (AA 1-321) (ABIN935101)
抗原
See all Thioredoxin Reductase (TrxR) 蛋白
Thioredoxin Reductase (TrxR)
蛋白类型
Recombinant
生物活性
Active
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 90 % pure
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产品特性
- AA 1-321
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序列
- MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K
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产品特性
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Purified recombinant E.coli TRXB protein
Expression System: E.coli
Bioactivity: Specific activity is 4-5 units/mL, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
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Thioredoxin Reductase (TrxR) (AA 1-321) protein
TrxR 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
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应用备注
- TRXB protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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实验流程
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- Prepare a 0.7 mL reaction mixture into a suitable container: The final concentrations are 107 mM potassium phosphate, 17 mM EDTA, 0.7 mM beta-NADPH, 0.05 % BSA, 0.014 % (w/v) thioredoxin, 7 mM DTNB, 5 µg, E. coli TRXB.
2. Equilibrate to 25 °C and monitor the A412 nm until the value is constant using a spectrophotometer.
3. Add 35 µL of 107 mM DTNB into reaction mixture and mix immediately.
4. Record the increase in A412 nm for 2 minutes.
- Prepare a 0.7 mL reaction mixture into a suitable container: The final concentrations are 107 mM potassium phosphate, 17 mM EDTA, 0.7 mM beta-NADPH, 0.05 % BSA, 0.014 % (w/v) thioredoxin, 7 mM DTNB, 5 µg, E. coli TRXB.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Thioredoxin Reductase (TrxR)
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别名
- TRXB
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背景
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TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidative stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: protein, Thioredoxin reductase protein, FAD/NAD(P)-binding protein, Thioredoxin reductase JW0871 protein, TRXR., ECK0879 protein -
分子量
- 34.6 kDa (321 AA)
抗原
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