电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

Glyoxalase Protein (AA 1-184)

This anti-Glyoxalase antibody (ABIN935017) is a Escherichia coli (E. coli) antibody detecting Glyoxalase in SDS. Suitable for Human.
产品编号 ABIN935017
发货至: 中国

Quick Overview for Glyoxalase Protein (AA 1-184) (ABIN935017)

抗原

Glyoxalase

蛋白类型

Recombinant

生物活性

Active

宿主

资源

大肠杆菌(E. Coli)

应用范围

SDS-PAGE (SDS)

纯度

> 90 % pure
  • 产品特性

    AA 1-184

    序列

    MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM

    产品特性

    Purified recombinant Human Glyoxalase? protein
    Expression System: E.coli
    Bioactivity: Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25 °C.
    Molecular weight on SDS-PAGE will appear higher.
  • 想要此Protein的其他选项吗?

    !
    探索我们的预定义定制蛋白和定制蛋白服务!

    您的项目需要进一步定制吗?联系我们,了解我们的定制蛋白解决方案

  • 应用备注

    Glyoxalase I protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

    实验流程

    1. Prepare a 1.5 mL reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033 % (w/v) reduced glutathine, 0.003 % (w/v) bovine serum albumin.
      2. Equilibrate to 25 °C and monitor at A240 nm (absorbance) until the value is constant, using a spectrophotometer.
      3. Add 50 µL of recombinant glyoxalase I solution with various concentrations (0.5 µg, 5 µg, 5 µg) in 1.5 mL reaction buffer.
      4. Immediately mix by inversion and record the increase at A240 nm for 5 minutes.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, and 10 % glycerol.

    储存液

    Dithiothreitol (DTT)

    注意事项

    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles.

    储存条件

    RT/-20 °C

    储存方法

    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • 抗原

    Glyoxalase

    别名

    Glyoxalase?

    背景

    Glyoxalase I, also known as GLO1, belongs to the glyoxalase family. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: GLO-1 Methylglyoxalase protein, Lactoylglutathione lyase protein, Lactoyl glutathione lyase protein, Ketone aldehyde mutase protein, Glx 1 protein, GLOD1 protein, S D lactoylglutathione methylglyoxal lyase 0., Glyoxalase I protein, GLYI protein, Glx I protein, Aldoketomutase protein

    分子量

    20.7 kDa (184 AA)
You are here:
Chat with us!