Glyoxalase Protein (AA 1-184)
This Recombinant Glyoxalase protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for Glyoxalase Protein (AA 1-184) (ABIN935017)
抗原
Glyoxalase
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 90 % pure
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产品特性
- AA 1-184
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序列
- MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
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产品特性
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Purified recombinant Human Glyoxalase? protein
Expression System: E.coli
Bioactivity: Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25 °C.
Molecular weight on SDS-PAGE will appear higher.
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应用备注
- Glyoxalase I protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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实验流程
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- Prepare a 1.5 mL reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033 % (w/v) reduced glutathine, 0.003 % (w/v) bovine serum albumin.
2. Equilibrate to 25 °C and monitor at A240 nm (absorbance) until the value is constant, using a spectrophotometer.
3. Add 50 µL of recombinant glyoxalase I solution with various concentrations (0.5 µg, 5 µg, 5 µg) in 1.5 mL reaction buffer.
4. Immediately mix by inversion and record the increase at A240 nm for 5 minutes.
- Prepare a 1.5 mL reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033 % (w/v) reduced glutathine, 0.003 % (w/v) bovine serum albumin.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, and 10 % glycerol.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- RT/-20 °C
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储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Glyoxalase
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别名
- Glyoxalase?
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背景
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Glyoxalase I, also known as GLO1, belongs to the glyoxalase family. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: GLO-1 Methylglyoxalase protein, Lactoylglutathione lyase protein, Lactoyl glutathione lyase protein, Ketone aldehyde mutase protein, Glx 1 protein, GLOD1 protein, S D lactoylglutathione methylglyoxal lyase 0., Glyoxalase I protein, GLYI protein, Glx I protein, Aldoketomutase protein -
分子量
- 20.7 kDa (184 AA)
抗原
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