Quick Overview for PPIH Protein (AA 1-177) (ABIN934991)
抗原
See all PPIH 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 1-177
-
序列
- MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM
-
产品特性
-
Purified recombinant Human PPIH protein
Expression System: E.coli
Bioactivity: Specific activity is > 40 nmoles/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-HCl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher. -
内毒素水平
- < 1.0 EU per µg of protein (determined by LAL method)
-
-
Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH) (AA 2-177) protein (His tag)
PPIH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH) (AA 1-177) protein (GST tag)PPIH 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH) (AA 1-177) proteinPPIH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH) protein (Myc-DYKDDDDK Tag)PPIH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
应用备注
- PPIH protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
-
实验流程
-
- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
2. Add recombinant Cyclophilin H protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
5. Record the increase in A410 nm for 20 minutes
- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Supplied as a liquid in PBS, pH 7.4, containing 10 % glycerol.
-
注意事项
- Avoid repeated freeze/thaw cycles.
-
储存条件
- RT/-20 °C
-
储存方法
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
-
-
- PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))
-
别名
- PPIH
-
背景
-
Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the U4/U6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: Peptidylprolyl isomerase H CypH protein, EC 5.2.1.8 protein, PPIH protein, UsnRNP associated cyclophilin SnuCyp20 protein, Peptidylprolyl isomerase H protein, Cyclophilin H protein, , CYPH protein, MGC5016 protein, CYP20 protein, USACYP protein, PPIase H protein, Rotamase H protein, SnuCyp-20 protein, SnuCyp-20 protein, Small nuclear ribonucleoprotein particle-specific cyclophilin H protein -
分子量
- 19.2 kDa (177 AA)
抗原
-
