Purified recombinant Human Stabilized Troponin I protein (Cardiac) Expression System: E.coli
应用备注
Each Investigator should determine their own optimal working dilution for specific applications.
限制
仅限研究用
浓度
10-50 µg/mL
缓冲液
Supplied in 500 mM NaCl, 5 mM sodium phosphate, 2 mM CaCl2, 0.5 mM EDTA, 0.025 % Sodium Azide, pH 7.5.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
-20 °C
储存方法
Store at -25 °C or below.
抗原
Stabilized Troponin I
背景
Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle. Alternative Names: Cardiac-specific troponin I protein, cTnl protein, TnI protein, Troponin I protein