IgE 蛋白
(3 references)Quick Overview for IgE 蛋白 (ABIN930022)
抗原
See all IgE 蛋白宿主
资源
应用范围
纯度
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产品特性
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Purified Human IgE protein
Source: Human plasma
Alternative Names: Immunoglobulin E Protein, Human IgE -
纯化方法
- Purified
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IgE (AA 208-429) protein (His-Avi Tag)
宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 209-428) protein (His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 91-421) protein (His tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 198-421) protein (His-Avi Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE protein宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE protein (Biotin)宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE (AA 1-429) protein (His tag)宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-428) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-432) protein (His tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 15 mM KH5 O4, pH 7.4, with 155 M NaCl and 0.05 % NaN3.
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20 °C.
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: "Aptamer-Au NPs conjugates-enhanced SPR sensing for the ultrasensitive sandwich immunoassay." in: Biosensors & bioelectronics, Vol. 25, Issue 1, pp. 124-9, (2009) (PubMed).
: "Optimization of aptamer microarray technology for multiple protein targets." in: Analytica chimica acta, Vol. 564, Issue 1, pp. 82-90, (2007) (PubMed).
: "Capture assay for specific IgE. An improved quantitative method." in: Journal of immunological methods, Vol. 157, Issue 1-2, pp. 65-72, (1993) (PubMed).
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: "Aptamer-Au NPs conjugates-enhanced SPR sensing for the ultrasensitive sandwich immunoassay." in: Biosensors & bioelectronics, Vol. 25, Issue 1, pp. 124-9, (2009) (PubMed).
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- IgE
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背景
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IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 mg/mL of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.
Synonyms: Immunoglobulin E Protein, Human IgE. -
分子量
- 200 kDa
抗原
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