Vimentin Protein (VIM) (AA 1-466)
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Quick Overview for Vimentin Protein (VIM) (AA 1-466) (ABIN8057080)
抗原
See all Vimentin (VIM) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-466
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原理
- Recombinant Human VIM Protein(Halo Tag)
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序列
- Met1-Glu466
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Vimentin (VIM) (AA 1-466) protein (GST tag)
VIM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vimentin (VIM) (AA 41-140) protein (GST tag)VIM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vimentin (VIM) (AA 2-201) protein (His tag)VIM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vimentin (VIM) (AA 2-466) protein (His-IF2DI Tag)VIM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Vimentin (VIM) (AA 2-466) protein (His tag)VIM 宿主: 人 宿主: 酵母菌 Recombinant > 90 % ELISA
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Vimentin (VIM)
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别名
- VIM
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背景
- Vimentin,Vimentin is a type III intermediate filament(IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl(tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.
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分子量
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Calculated MW: 93.1 kDa
Observed MW: 91 kDa
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基因ID
- 7431
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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