MMP1 Protein (AA 20-469)
Our Local Distributor
北京 101111
Quick Overview for MMP1 Protein (AA 20-469) (ABIN8057036)
抗原
See all MMP1 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 20-469
-
原理
- Recombinant Human MMP-1 Protein(Halo Tag)
-
序列
- Phe20-Asn469
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 1.0 EU/mg of the protein as determined by the LAL method
-
Biological Activity Comment
- Not validated for activity
-
-
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) (AA 100-469) protein (His tag)
MMP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) (AA 23-215) protein (His tag)MMP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) (AA 1-469) protein (GST tag)MMP1 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
-
缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
-
别名
- MMP-1
-
背景
- Collagenase 1,Interstitial collagenase,MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. MMP activity is regulated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. MMP-1,-2,-3,-7,-9,-13 and-14 all have elevated expression in primary tumors and/or metastases. MMP-1 cleaves collagens of types I, II, and III at one site in the helical domain. It also cleaves collagens of types VII and X. In case of HIV infection, MMP1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
-
分子量
-
Calculated MW: 83.3 kDa
Observed MW: 80 kDa
-
基因ID
- 4312
-
UniProt
- P03956
抗原
-
