YTHDC1 Protein (full length)
Our Local Distributor
北京 101111
Quick Overview for YTHDC1 Protein (full length) (ABIN7939762)
抗原
See all YTHDC1 蛋白蛋白类型
宿主
资源
应用范围
-
-
产品特性
- full length
-
原理
- Recombinant YTHDC1 protein
-
产品特性
- Recombinant YTHDC1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_001026902.1) with an N-terminal Flag tag. The molecular weight of the protein is 85.6 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
-
-
YTH Domain Containing 1 (YTHDC1) (AA 1-709) protein (GST tag)
YTHDC1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Recombinant YTHDC1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
-
注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
-
储存条件
- -80 °C
-
储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
-
-
- YTHDC1 (YTH Domain Containing 1 (YTHDC1))
-
别名
- YTHDC1
-
背景
-
Protein Background: YTHDC1 (YTH Domain Containing 1), is a regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDC1 acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. It specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. YTHDC1 may also regulate alternative splice site selection.
Short Description: Recombinant YTHDC1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001026902.1) with an N-terminal FLAG tag. The molecular weight of the protein is 85.6 kDa.
Aliases: YTH Domain Containing 1, Splicing Factor YT521
-
分子量
- 85.6 kDa
-
NCBI登录号
- NP_001026902
抗原
-
