UBE2N 蛋白
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北京 101111
Quick Overview for UBE2N 蛋白 (ABIN7939713)
抗原
See all UBE2N 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant UBE2N protein
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 116,946.14 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (His tag)
custom-made UBE2N 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (GST tag)UBE2N 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (His tag)UBE2N 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 1-152) protein (GST tag)UBE2N 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2N (UBE2N) (AA 2-152) protein (His-IF2DI Tag)UBE2N 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2N (UBE2N) protein (Myc-DYKDDDDK Tag)UBE2N 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant UBE2N protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))
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别名
- UBE2N
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背景
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Protein Background: UBE2N (Ubiquitin-conjugating enzyme E2 N)also known as HEL-S-71, UBC13, UbcH-ben, UbcH13 and UBCHBEN. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production.
Short Description: Recombinant UBE2N protein that includes full length of human UBE2N protein (accession number NP_003339.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 19.30 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiquitin conjugating enzyme E2 N, HEL-S-71, UBC13, UbcH-Ben, UbcH13, UBCHBEN
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway
抗原
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